3. reagents used within the histology laboratory are available both within the laboratory and in the preparation room (C222). You may modify each section in the space provided at the bottom of each page. 9 HISTOPATHOLOGY LABORATORY Histopathology laboratory is a unit performing clinical activity associated with diagnosis during and post-surgery. Laboratory Procedure Manual on histopathology – Mohs surgery and modify or rewrite to conform with the practices in your laboratory. 5. Choose appropriate inpatient surgical request card or outpatient histopathology specimen requisition and fill in completely (if applicable). Wash in tap water, rinse in distilled. 9.1: Rotary Microtome Fig. 6. 1% Sodium metabisulfite, 1 minute, room temperature. 18 Record of Changes I. histopathology techniques. A number of histologic stains are available that are routinely used to visualize fungi in tissue sections. HISTOLOGY AND CYTOLOGY 51 MODULE Microtome Histology and Cytology Notes Fig. procedure or unexpected findings Example: “possible positive deep margin” or “possible neoplasm” o Place the Pathology Tissue Slip in the side pocket of the biohazard bag along with the insurance information sheet • For specimens ordered online: 1. This textbook deals with basic histopathology techniques: Introduction and examination of tissue, fixation and fixatives, tissue processing and … Deparaffinize and hydrate to distilled water. *2% Chromic acid, microwave Hi power, 45 seconds, allow to stand for 5 minutes. Clearing c. Infilteration 7. 1. Muscle Biopsies (for detailed instructions see Clinical Guidelines, GOS Web) All muscle biopsies should be pre-booked with histopathology. 9.2: Principle of sample movement for making a cut on a rotary microtome In the figure to the left, the principle of the cut is explained. To Outline the Proper Procedures for Histopathology . Histology Procedure Fixation Tissue Processing a. Dehydration b. To outline the proper procedures for collecting, preserving, identifying, and processing tissue specimens for evaluation by light microscopic histopathological evaluation. 2. histopathology is the only way to diagnose infections caused by L. loboi or Rhinosporidium seeberi [Figure 2] as cultivation techniques have to date been unsuccessful. RESPONSIBILITY . Tissue Fixation Tissues are fixed: •To preserve cells and tissues constituents •To prevent their degradation Bouin’s fixative/formalin for 24 hrs (with fixative’s volume 20 times greater) 6. HISTOLOGY ATC BRANCH HD-19-HSC-10.2 HISTOLOGY SPECIMEN COLLECTION Effective: 2/18/13, Rev 3/4/2014 9. Histology Procedure Fixation 5. When ‘bringing sections to water’ or ‘dehydrating, clearing and mounting’ always 5.7 Staining procedures Whenever doing staining procedures ensure that protective gowns, gloves and safety glasses are worn. 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